Antonie van Leeuwenhoek made high-quality small lenses and used simple microscopes to examine water droplets, dental plaque, and other samples. He reported the microorganisms he observed to the scholarly community.
Many of his instruments used a single lens. A microscope's value depends not only on its complexity but also on lens quality and the observer's skill.
Microscopic study
While running his draper shop, Van Leeuwenhoek wanted to see the quality of the thread better than what was possible using the magnifying lenses of the time. He developed an interest in lensmaking, although few records exist of his early activity. By placing the middle of a small rod of soda lime glass in a hot flame, one can pull the hot section apart to create two long whiskers of glass. Then, by reinserting the end of one whisker into the flame, a very small, high-quality glass lens is created. Significantly, a May 2021 neutron tomography study of a high-magnification Leeuwenhoek microscope captured images of the short glass stem characteristic of this lens creation method. For lower magnifications he also made ground lenses. To help keep his methods confidential he apparently intentionally encouraged others to think grinding was his primary or only lens construction method.
Recognition by the Royal Society
After developing his method for creating powerful lenses and applying them to the study of the microscopic world, Van Leeuwenhoek introduced his work to his friend, the prominent Dutch physician Reinier de Graaf. When the Royal Society in London published the groundbreaking work of an Italian lensmaker in their journal Philosophical Transactions of the Royal Society, de Graaf wrote to the editor of the journal, Henry Oldenburg, with a ringing endorsement of Van Leeuwenhoek's microscopes which, he claimed, "far surpass those which we have hitherto seen". In response, in 1673 the society published a letter from Van Leeuwenhoek that included his microscopic observations on mold, bees, and lice. Then, in 1674, Van Leeuwenhoek made his most significant discovery. Starting from the assumption that life and motility are similar, he determined that the moving objects observed under his microscope were little animals. He later recorded his observations in his diary.
He was also the first to use the word animalcules to translate the Dutch words that Leeuwenhoek used to describe microorganisms. Despite the initial success of Van Leeuwenhoek's relationship with the Royal Society, soon relations became severely strained. His credibility was questioned when he sent the Royal Society a copy of his first observations of microscopic single-celled organisms dated 9 October 1676. Previously, the existence of single-celled organisms was entirely unknown. Thus, even with his established reputation with the Royal Society as a reliable observer, his observations of microscopic life were initially met with some skepticism.
Techniques
Antonie van Leeuwenhoek made more than 500 optical lenses. He also created at least 25 single-lens microscopes, of differing types, of which only nine have survived. These microscopes were made of silver or copper frames, holding hand-made lenses. Those that have survived are capable of magnification up to 275 times. It is suspected that Van Leeuwenhoek possessed some microscopes that could magnify up to 500 times. Although he has been widely regarded as a dilettante or amateur, his scientific research was of remarkably high quality.
The single-lens microscopes of Van Leeuwenhoek were relatively small devices, the largest being about 5 cm long. They are used by placing the lens very close in front of the eye. The other side of the microscope had a pin, where the sample was attached in order to stay close to the lens. There were also three screws to move the pin and the sample along three axes: one axis to change the focus, and the two other axes to navigate through the sample.
Van Leeuwenhoek maintained throughout his life that there were aspects of microscope construction "which I only keep for myself", in particular his most critical secret of how he made the lenses. For centuries, Van Leeuwenhoek's exact method remained unknown. In 1953, a procedure was published to build a working replica, creating the lens by inserting a thin glass thread into a flame as described above. In May 2021, researchers in the Netherlands published a neutron tomography study of Leeuwenhoek's original microscopes, revealing a spherical lens with a short stem attached. This stem was the remaining section of the glass filament used, confirming the method of manufacture. As noted there, this method was originally devised by Robert Hooke rather than Leeuwenhoek, which is ironic given Hooke's subsequent surprise at Leeuwenhoek's findings.